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Adiponectin Increases Glucose-Dependent Electrical Activity and Insulin Secretion in Mouse Pancreatic ß Cells
- Source :
- Diabetes. 67
- Publication Year :
- 2018
- Publisher :
- American Diabetes Association, 2018.
-
Abstract
- Decreased plasma adiponectin has been implicated as a cause of diabetes. However, the functional impact of adiponectin on glucose-dependent responses of β-cells remains poorly understood. Here we have investigated whether chronically and acutely increased adiponectin levels affect electrical activity (sharp electrodes) and insulin secretion in β-cells using respectively, adiponectin overexpressing (APNtg) mice and acute application of adiponectin. In the acute exposure experiments, adiponectin (20 µg/ml, 11 mM glucose) increased insulin secretion 42% and β-cell electrical activity (measured as the fraction ‘active phase’) 32%. In islets from APNtg mice insulin secretion increased 44% and active phase increased 25%. The effects on electrical activity in APNtg β-cells correlated with membrane hyperpolarization and the resting membrane potential at 0 mM glucose averaged -60±2 in control and -69±2 in APNtg β-cells. There was a moderate effect on action potential width ( Disclosure J.I. Real: None. B. Chanclon Garcia: None. I. Wernstedt Asterholm: None. P. Rorsman: None.
- Subjects :
- Membrane potential
medicine.medical_specialty
geography
geography.geographical_feature_category
Adiponectin
Chemistry
Endocrinology, Diabetes and Metabolism
Membrane hyperpolarization
Islet
medicine.disease
Endocrinology
Acute exposure
Internal medicine
Active phase
Diabetes mellitus
Internal Medicine
medicine
Insulin secretion
Subjects
Details
- ISSN :
- 1939327X and 00121797
- Volume :
- 67
- Database :
- OpenAIRE
- Journal :
- Diabetes
- Accession number :
- edsair.doi...........6063c7c5ceea6169d70852da9c3fdc4d