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Irradiated and CCl 4 ‐treated bone marrow‐derived liver macrophages exhibit different gene expression patterns and phenotypes
- Source :
- Scandinavian Journal of Immunology. 92
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Myeloid cells infiltrate into the liver and differentiate into macrophages in different liver injury mouse models. However, the heterogeneity of bone marrow (BM)-derived LMs populations remains to be understood. To investigate this and understand the impact of the macrophage niche on the properties of recruited BM-derived macrophages, we used a non-myeloablation BM transplantation model to label and trace BM-derived LMs. Subsequently, we quantified the number of embryonic-derived liver-resident macrophages, BM-derived LMs and total LMs in CCl4 and irradiated acute liver injury mouse models, respectively. Finally, we compared the cell fate, gene expression patterns, chemokine signals, and surface markers of irradiated and CCl4 -treated BM-derived LMs. We observed that, as compared to CCl4, radiation generated a macrophage niche by depleting embryonic-derived liver-resident macrophages and induced the recruitment of BM-derived LMs that further settled in the liver. Irradiated and CCl4 -treated BM-derived LMs are different with respect to their cell fates, gene expression patterns, and chemokine expression and recruitment. They also have different surface markers shortly after differentiating from their progenitors. Our findings suggest that irradiated and CCl4 -treated LM populations derived from the bone marrow display different patterns of gene expression and phenotypes; these differences may be due to the availability of macrophage niche.
- Subjects :
- 0301 basic medicine
Liver injury
Chemokine
biology
Immunology
CCL4
General Medicine
medicine.disease
Cell biology
Transplantation
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
medicine.anatomical_structure
Gene expression
medicine
biology.protein
Macrophage
Bone marrow
Progenitor cell
030215 immunology
Subjects
Details
- ISSN :
- 13653083 and 03009475
- Volume :
- 92
- Database :
- OpenAIRE
- Journal :
- Scandinavian Journal of Immunology
- Accession number :
- edsair.doi...........5fb433d5dd5261757ed4c3b8fe58d56f