218 AN EVALUATION OF IN VITRO MATURATION OOCYTE ACTIVATION METHODS FOR IMPROVING OVINE INTRACYTOPLASMIC SPERM INJECTION EFFICIENCY

Given previous low sperm decondensation rates and poor oocyte activation in sheep ICSI (10–20%), we evaluated activation techniques for IVM/ICSI. Incubations were performed in a 5% CO2 cell incubator at 38.5°C and saturated humidity. Sheep ovaries were collected at an abattoir and transported