Engineering of microbial cellulases for value-added product generations

Lignocellulosic biomass is extensively present as cheap-cost recyclable resource on the globe. Lignocellulosic biomass transformation toward value-added biomolecules is the most sustainable approach as it is not competing with food chain or feed cycle. Lignocellulosic biomass conversion to value-added products is being carried out following various existing routes such as thermochemical, chemical, and or biological avenues. However, biological route of lignocellulosic biomass conversation via enzymes is one the most feasible, rapid, and ecological benign approach. Diverse range of microbial communities have been involved to secret various extracellular cellulase enzymes that degrade stubborn lignocellulosic biomass into simple carbon sources, which can further be converted into value-added products starting from biofuel to bioplastics. However, catalytic activities of these cellulases are not up to the mark that can support industrial need at present due to lower substrate specificity, substrate conversion rate, and turnover number. To this end, many experimental analysis have already been carried out to improve the bioprocess development for enhancing lignocellulosic biomass degradation through engineering (mutations and genetic engineering) intervention on cellulase enzymes. Hence, this chapter will depict a critical overview on current research activities toward ameliorating cellulase enzyme catalytic efficacy through mutational, genetic, and protein engineering approaches for lignocellulosic biomass conversation to value-added products.