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Large-Volume Sample Staking of Rice Polyphenols Prior to Their Determination by Non-aqueous Capillary Electrophoresis
- Source :
- Food Analytical Methods. 9:2152-2160
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- A rapid non-aqueous capillary electrophoretic (NACE) method for the separation of (−)-epicatechin (EPI), (+)-catechin (−CAT), kaempferol (KAE), quercetin (QUR), naringanin (NAR), ferulic acid (FA), and p-coumaric acid (p-CA) has been developed and applied to the determination of these compounds in different rice varieties. All seven compounds were separated on capillary of 50 μm × 68 cm (60-cm effective length) using 20 mmol L−1 borate buffer of pH 9.0 and 5 % acetonitrile in methanol. Large-volume sample stacking (LVSS) technique was optimized and used to preconcentrate non-detectable polyphenols of white polished rice. Rice extracts were concentrated on-line by LVSS prior to separation by non-aqueous capillary electrophoresis. An improvement of 10–55 times in detectability was achieved with injection at 50 mbar for 30 s followed by voltage inversion (−20 kV) for 5 s. Linear calibration range of 1–300 μg L−1 and 0.01–60 μg L−1 was observed for NACE and NACE-LVSS method respectively, with the detection limit of 0.33–2.0 and 0.006–0.19 μg L−1. Good reproducibility with standard deviations of less than 5 % was achieved. Polyphenol contents of different rice varieties were determined using developed method.
- Subjects :
- Detection limit
Chromatography
Aqueous solution
010401 analytical chemistry
02 engineering and technology
021001 nanoscience & nanotechnology
01 natural sciences
Applied Microbiology and Biotechnology
0104 chemical sciences
Analytical Chemistry
Ferulic acid
chemistry.chemical_compound
Electrophoresis
Capillary electrophoresis
chemistry
Polyphenol
Methanol
0210 nano-technology
Safety, Risk, Reliability and Quality
Kaempferol
Safety Research
Food Science
Subjects
Details
- ISSN :
- 1936976X and 19369751
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Food Analytical Methods
- Accession number :
- edsair.doi...........5eb08989e168db42020179abcbffd5f6