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Preparation of nanogel-immobilized porous gel beads for affinity separation of proteins: fusion of nano and micro gel materials
Preparation of nanogel-immobilized porous gel beads for affinity separation of proteins: fusion of nano and micro gel materials
- Source :
- Polymer Journal. 47:220-225
- Publication Year :
- 2014
- Publisher :
- Springer Science and Business Media LLC, 2014.
-
Abstract
- Nanogel particles (NP) that bind with the Fc fragment of immunoglobulin G (IgG) were immobilized on the pore surface of macroporous hard gel beads (GB) containing quaternary ammonium cations on the surface via multipoint electrostatic interactions. The model target protein (IgG) was reversibly captured by the NP-immobilized GBs through NP–IgG interactions. NP-immobilized GBs have potential applications as a novel affinity purification medium for proteins, combining an inexpensive and stable ligand with a high-performance support.
Details
- ISSN :
- 13490540 and 00323896
- Volume :
- 47
- Database :
- OpenAIRE
- Journal :
- Polymer Journal
- Accession number :
- edsair.doi...........5b6b1d0c7b66d8503d6557d31c9b9ff4
- Full Text :
- https://doi.org/10.1038/pj.2014.101