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Preparation of nanogel-immobilized porous gel beads for affinity separation of proteins: fusion of nano and micro gel materials

Preparation of nanogel-immobilized porous gel beads for affinity separation of proteins: fusion of nano and micro gel materials

Authors :
Kenneth J. Shea
Yuka Arata
Yoshiko Miura
Yusuke Yonamine
Katsuhiko Yano
Ryousuke Tsuhara
Aki Yamasaki
Yu Hoshino
Shih Hui Lee
Source :
Polymer Journal. 47:220-225
Publication Year :
2014
Publisher :
Springer Science and Business Media LLC, 2014.

Abstract

Nanogel particles (NP) that bind with the Fc fragment of immunoglobulin G (IgG) were immobilized on the pore surface of macroporous hard gel beads (GB) containing quaternary ammonium cations on the surface via multipoint electrostatic interactions. The model target protein (IgG) was reversibly captured by the NP-immobilized GBs through NP–IgG interactions. NP-immobilized GBs have potential applications as a novel affinity purification medium for proteins, combining an inexpensive and stable ligand with a high-performance support.

Details

ISSN :
13490540 and 00323896
Volume :
47
Database :
OpenAIRE
Journal :
Polymer Journal
Accession number :
edsair.doi...........5b6b1d0c7b66d8503d6557d31c9b9ff4
Full Text :
https://doi.org/10.1038/pj.2014.101