Analysis of Synonymous Codon Usage Bias in Pseudomonas Syringae Phages: Implication in Phage Therapy for Halo Blight Disease

Halo blight disease caused due Pseudomonas syringae results extensive losses in dry Beans. Phage therapy can be tried as an alternative treatment. For evaluation of synonymous codon usage and codon variation, 20 phages of Pseudomonas has been taken for testing. The effect of GC concentration in the phages can be considered for analyzing virulence in these phages. Mutational biasing and translational selection are also the important factors for predicting the appropriate biasing, which can be analyzed through Nc plot and correspondence analysis. Our analysis indicating that out of 20 phages 3 phages namely phage D3, D3112 and 119X are extremely virulent as they have high translational efficiency. Based on our data, we conclude that the phage D3 will be best suited as a phage therapy for treatment against halo blight disease.