REDUCED EXPRESSION OF MIR-150 LEADS TO IMPAIRMENT OF ISCHEMIA-INDUCED NEOVASCULARISATION IN HYPERCHOLESTEROLEMIC CONDITIONS

s S277 treatment of HC mice with miR-150 mimic can rescue the number of bone marrow and peripheral EPCs. Moreover, the angiogenic properties of EPCs (adhesion, migration and integration into tubules) are restored in HC mice treated with miR-150 mimic. In vitro, miR-150 rescues angiogenesis (migration, tube formation) in HUVECs exposed to oxLDL. miR-150 has previously been shown to target SRC kinase signaling inhibitor 1 (SRCIN1), an important regulator of SRC activity. We found that SRC activity is significantly reduced in HUVECs exposed to oxLDL. Moreover, the angiogenic effects of miR-150 mimic in HUVECs exposed to oxLDL are completely abolished when the cells are also treated with an inhibitor of SRC. CONCLUSION: Hypercholesterolemia is associated with reduced expression of miR-150, which leads to impaired neovascularization following ischemia. The mechanism involves beneficial effects on both angiogenesis and EPC activities. Our results suggest that SRC signaling is at least in part responsible for the pro-angiogenic effects of miR-150 in these conditions. IRSC 525 NEONATAL EXPOSURE TO HIGH OXYGEN LEVELS IS ASSOCIATED WITH IMPAIRED ISCHEMIA-INDUCED NEOVASCULARIZATION DURING ADULTHOOD R Mathieu, M Desjarlais, W Dhahri, A Cloutier, A Nuyt, A Rivard