Functional Analysis of Transgenic Cry1Iem Maize

The genetic transformation of maize callus was induced by Agrobacterium tumefaciens. The Cry1Iem was transferred into callus of inbred line H99 maize, and the transgenic plants were detected by herbicide screening and PCR. A further Southern blotting experiment, real- time quantitative PCR and indoor/outdoor insect resistance identification test were carried out on T2 transgenic plants. The results of Southern blotting of transgenic plants showed that Cry1Iem had been successfully integrated into the maize genome. Real-time quantitative PCR showed that Cry1Iem was successfully expressed in transgenic plants. The insect resistance test showed that the resistance of the transgenic plants was significantly improved.