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Anti-Inflammatory Activity of Antimicrobial Peptide Allomyrinasin Derived from the Dynastid Beetle, Allomyrina dichotoma
- Source :
- Journal of Microbiology and Biotechnology. 29:687-695
- Publication Year :
- 2019
- Publisher :
- Journal of Microbiology and Biotechnology, 2019.
-
Abstract
- In a previous work, we performed de novo RNA sequencing of Allomyrina dichotoma using next generation sequencing and identified several antimicrobial peptide candidates based on transcriptome analysis. Among them, a cationic antimicrobial peptide, allomyrinasin, was selected bioinformatically based on its physicochemical properties. Here, we assessed the antimicrobial and anti-inflammatory activities of allomyrinasin against microorganisms and mouse macrophage Raw264.7 cells. Allomyrinasin showed antimicrobial activities against various microbes and decreased the nitric oxide production of the lipopolysaccharide-induced Raw264.7 cells. Furthermore, quantitative RT-PCR and ELISA revealed that allomyrinasin reduced cytokine expression levels in the Raw264.7 cells. We also identified inducible nitric oxide synthase, cyclooxygenase-2 expression, and PGE2 production through western blot analysis and ELISA. We confirmed that allomyrinasin bound to bacterial cell membranes via a specific interaction with lipopolysaccharides. Taken together, these data indicate that allomyrinasin has antimicrobial and anti-inflammatory activities as exemplified in lipopolysaccharide-induced Raw264.7 cells. We have provided a potentially useful antimicrobial peptide candidate that has both antimicrobial and anti-inflammatory activities.
- Subjects :
- 0106 biological sciences
chemistry.chemical_classification
biology
medicine.diagnostic_test
Lipopolysaccharide
medicine.drug_class
Chemistry
RNA
Peptide
General Medicine
Antimicrobial
01 natural sciences
Applied Microbiology and Biotechnology
Anti-inflammatory
Microbiology
Nitric oxide synthase
Transcriptome
chemistry.chemical_compound
Western blot
010608 biotechnology
biology.protein
medicine
Biotechnology
Subjects
Details
- ISSN :
- 17388872 and 10177825
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- Journal of Microbiology and Biotechnology
- Accession number :
- edsair.doi...........59ca7b9d3ef929d275d68f96a1276dea