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Genome-Wide Study of mRNA Isoform Half-Lives

Authors :
Zarmik Moqtaderi
Joseph V. Geisberg
Source :
Methods in Molecular Biology ISBN: 9781493930661
Publication Year :
2016
Publisher :
Springer New York, 2016.

Abstract

In eukaryotes, RNA polymerase II-driven transcription and processing results in the formation of numerous mRNA 3' isoforms that for any given gene may differ from one another by as little as a single nucleotide. These 3' isoforms can vary in physical properties that may affect their function and stability. Here, we outline a systematic framework to measure individual mRNA 3' isoform half-lives on a genome-wide level in S. cerevisiae. Our approach utilizes the Anchor-Away system to sequester RNA polymerase II (Pol II) in the cytoplasm followed by direct single-molecule RNA sequencing to generate a highly detailed view of 3' isoform stability under most physiological conditions without many of the adverse effects associated with commonly used alternative approaches.

Details

ISBN :
978-1-4939-3066-1
ISBNs :
9781493930661
Database :
OpenAIRE
Journal :
Methods in Molecular Biology ISBN: 9781493930661
Accession number :
edsair.doi...........57e2ca83216900976ba084f2065561a6
Full Text :
https://doi.org/10.1007/978-1-4939-3067-8_20