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Toxicological evaluation of aspartame against Madin–Darby canine kidney cells
- Source :
- Journal of Food Measurement and Characterization. 11:355-363
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- Aspartame is most widely used as artificial sweeteners in more than 6000 food varieties. Aspartame digested into aspartic acid, phenylalanine, and methanol, and several peroxides, superoxide molecules also generated together. The kidney is the secondary site for the cellular metabolism. The present study examined whether the treatment of aspartame induces oxidative stress in the Madin–Darby kidney cells (MDCK). The effects of aspartame on MDCK cell viability were investigated by the sulphorhodamine-B assay and flow cytometry. Morphology of MDCK cells following aspartame exposure was observed. Mitochondria-derived reactive oxygen species (ROS) was also determined using 2′,7′-dichlorodihydrofluorescein diacetate. Lipid peroxidation (LPO), glutathione reduced (GSH) levels and activities of superoxide dismutase (SOD), and catalase enzymes were also determined. Cell viability was significantly altered following aspartame exposure. Morphology of MDCK cells did not change significantly. However, there was a marginal morphological change, including rounding, sporadic distribution and loss of adherence were observed at higher doses of aspartame exposure. Mitochondria-derived ROS was increased in a dose-dependent manner following aspartame exposure. A significant increase in LPO levels, whereas GSH level was reduced after 48 and 72 h of aspartame exposure. SOD and catalase enzyme activities were significantly reduced in a dose- and time-dependent manner. Taking all these data together, it is concluded that aspartame may induce oxidative stress in the MDCK cells.
- Subjects :
- 0301 basic medicine
chemistry.chemical_classification
Reactive oxygen species
biology
Aspartame
General Chemical Engineering
Phenylalanine
Glutathione
medicine.disease_cause
Industrial and Manufacturing Engineering
Lipid peroxidation
Superoxide dismutase
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
chemistry
Biochemistry
Catalase
biology.protein
medicine
Safety, Risk, Reliability and Quality
Oxidative stress
Food Science
Subjects
Details
- ISSN :
- 21934134 and 21934126
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Journal of Food Measurement and Characterization
- Accession number :
- edsair.doi...........57e2906d74c86ed5fa28565b370e2aa1