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Efficient isolation of rare B cells using next-generation antigen barcoding

Authors :
Jonathan Hurtado
Claudia Flynn
Jeong Hyun Lee
Eugenia Salcedo
Christopher A. Cottrell
Patrick D. Skog
David Nemazee
William R. Schief
Elise Landais
Devin Sok
Bryan Briney
Publication Year :
2022
Publisher :
Cold Spring Harbor Laboratory, 2022.

Abstract

The ability to efficiently isolate antigen-specific B cells in high throughput will greatly accelerate the discovery of therapeutic monoclonal antibodies (mAbs) and catalyze rational vaccine development. Traditional mAb discovery is a costly and labor-intensive process, although recent advances in single-cell genomics using emulsion microfluidics allow simultaneous processing of thousands of individual cells. Here we present a streamlined method for isolation and analysis of large numbers of antigen-specific B cells, including next generation antigen barcoding and an integrated computational framework for B cell multi-omics. We demonstrate the power of this approach by recovering thousands of antigen-specific mAbs, including the efficient isolation of extremely rare precursors of VRC01-class and IOMA-class broadly neutralizing HIV mAbs.

Details

Database :
OpenAIRE
Accession number :
edsair.doi...........5786a0d131d1965b5bc656ab1549fdd0
Full Text :
https://doi.org/10.1101/2022.06.06.495029