Further Applications of Diazotized 4-Amino-6-chloro-m-benzenedisulfonamide

The diazotization product of 4-amino-6-chloro-m-benzenedisulfonamide has been found to be a useful reagent for the colorimetric determination of a vitamin, steroid, and alkaloid. A general method, with slight modification, can be applied to those compounds which contain a phenolic or similar-to-phenolic hydroxyl group. Pyridoxine hydrochloride, 17β-hydroxy-7aα-17α-dimethyl-B-homo-A-norestrane-3,6-dione, and morphine sulfate were used as coupling components in this study. The optimum reaction conditions for coupling and color formation have been determined and the applicability of the method in the presence of commonly used ingredients is demonstrated.