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Detection of human papillomavirus infection in oral cancers reported at dental facility: assessing the utility of FFPE tissues

Authors :
Sukh Mahendra Singh
Dinesh Chandra Doval
Suhail Chhakara
Durgatosh Pandey
Urmi Sarkar
Ankit Goel
Kiran Agarwal
Abhishek Tyagi
Nikita Aggarwal
Kanchan Vishnoi
Alok C. Bharti
Ravi Mehrotra
Tejveer Singh
Mohit Jadli
Gaurav Verma
Shashi Sharma
Ankita Sharma
Source :
Medical Oncology. 39
Publication Year :
2021
Publisher :
Springer Science and Business Media LLC, 2021.

Abstract

Incidence of human papillomavirus (HPV)-associated oral cancers is on the rise. However, epidemiological data of this subset of cancers are limited. Dental hospital poses a unique advantage in detection of HPV-positive oral malignancies. We assessed the utility of formalin-fixed paraffin-embedded (FFPE) tissues, which are readily available, for evaluation of high-risk HPV infection in oral cancer. For protocol standardization, we used 20 prospectively collected paired FFPE and fresh tissues of histopathologically confirmed oral cancer cases reported in Oral Medicine department of a dental hospital for comparative study. Only short PCRs (~ 200 bp) of DNA isolated using a modified xylene-free method displayed a concordant HPV result. For HPV analysis, we used additional 30 retrospectively collected FFPE tissues. DNA isolated from these specimens showed an overall 23.4% (11/47) HPV positivity with detection of HPV18. Comparison of HPV positivity from dental hospital FFPE specimens with overall HPV positivity of freshly collected oral cancer specimens (n = 55) from three cancer care hospitals of the same region showed notable difference (12.7%; 7/55). Further, cancer hospital specimens showed HPV16 positivity and displayed a characteristic difference in reported sub-sites and patient spectrum. Overall, using a xylene-free FFPE DNA isolation method clubbed with short amplicon PCR, we showed detection of HPV-positive oral cancer in dental hospitals.

Details

ISSN :
1559131X and 13570560
Volume :
39
Database :
OpenAIRE
Journal :
Medical Oncology
Accession number :
edsair.doi...........56591ac3b58a48d870d285aa613bef1a
Full Text :
https://doi.org/10.1007/s12032-021-01608-5