Significance of fluorescent inter simple sequence repeat technique for distinguishing domestic animal breeds

Conservation of farm animal genetic resources is of fundamental importance for the study of the relationships among breeds. The aim of this study was to evaluate the usefulness of the nuclear fluorescence inter simple sequence repeat (FISSR) markers in order to shed light on the genetic biodiversity of domestic animals. Two modifications of the original technique were made so as to make it more suitable for routine needs. The modified FISSR protocol was tested on different breeds of goat and donkey from Sardinia, a Mediteranean island known for its biodiversity. The two species are affected by different management problems in Sardinia: goats need a traceability of local products from different breeds, whereas donkeys are drastically reduced in number. The primers used were found to be very informative suggesting that the modified FISSR can be successfully applied in studies on different breeds of animal species without expensive experimentations. This method could be of interest in many geographic regions where there are more breeds of the same species with similar morphological features and different genetic pattern. The strongest point of this method is its low cost. Key words: Fluorescence inter simple sequence repeat (FISSR), biodiversity, genetic variability, goat, donkey.