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Molecular breeding of sporeless strains of Pleurotus ostreatus using a non-homologous DNA end-joining defective strain
- Source :
- Mycological Progress. 20:73-81
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Gene targeting is useful to isolate strains with mutations in a gene of interest for efficient breeding. In this study, we generated msh4 or mer3 single-gene disruptant monokaryons using a Pleurotus ostreatus Δku80 strain for efficient gene targeting. Dikaryons of P. ostreatus Δmsh4×Δmsh4 or Δmer3×Δmer3 were isolated via backcrosses, and the number of basidiospores produced was measured. The number of basidiospores fell by an average 1/13.7 in the P. ostreatus Δmsh4×Δmsh4 dikaryons versus the P. ostreatus msh4+×Δmsh4 dikaryons, and 1/82.6 in the P. ostreatus Δmer3×Δmer3 dikaryons versus the P. ostreatus mer3+×Δmer3 dikaryons. To demonstrate the effects of ku80 disruption, P. ostreatus Δku80×Δku80 dikaryon strains were isolated and no significant effects on basidiospore production were observed. Fluorescence microscopy showed meiotic progression was arrested during prophase I in the msh4 or mer3 disruptants. To our knowledge, this is the first report on molecular breeding of sporeless strains in cultivated mushrooms using an efficient method for targeted gene disruption.
- Subjects :
- Genetics
Molecular breeding
0303 health sciences
biology
Basidiospore
Strain (chemistry)
030306 microbiology
Gene targeting
biology.organism_classification
Agricultural and Biological Sciences (miscellaneous)
03 medical and health sciences
Meiosis
Pleurotus ostreatus
Gene
Ecology, Evolution, Behavior and Systematics
030304 developmental biology
Dikaryon
Subjects
Details
- ISSN :
- 18618952 and 1617416X
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Mycological Progress
- Accession number :
- edsair.doi...........5415f7a3366003c92e31936207f8fa90