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Biocompatible glutathione-capped gold nanoclusters for dual fluorescent sensing and imaging of copper(II) and temperature in human cells and bacterial cells
- Source :
- Microchimica Acta. 183:2185-2195
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- Highly red luminescent gold nanoclusters (AuNCs) have been prepared at physiological temperature by using glutathione (GSH) as a stabilizing and capping agent in aqueous media. The resultant AuNCs have been characterized by photoluminescence and X-ray photoelectron spectroscopy, transmission electron microscopy and FTIR. The AuNCs exhibit red fluorescence with a quantum yield of 3.1 %, and fluorescence is strongly quenched by copper(II) ion due to aggregation. This finding is exploited in a fluorometric assay for Cu(II) that has a 2.8 nM detection limit. The method was applied to the determination of Cu(II) ion in (spiked) mineral water and Tai Lake water. In addition, the AuNCs display a strongly temperature-dependent emission that can be used for optical determination of temperature in the 0 to 60 °C range. We finally show that the AuNCs are viable nanomaterials for (a) plain imaging of Hep-2 cells by laser scanning confocal microscopy, and for (b) intracellular imaging of Cu(II) and of temperature in Hep-2 cells and E. coli.
- Subjects :
- Chemistry
Analytical chemistry
chemistry.chemical_element
02 engineering and technology
010402 general chemistry
021001 nanoscience & nanotechnology
01 natural sciences
Fluorescence
Copper
0104 chemical sciences
Analytical Chemistry
Nanomaterials
law.invention
Nanoclusters
X-ray photoelectron spectroscopy
Transmission electron microscopy
Confocal microscopy
law
0210 nano-technology
Luminescence
Nuclear chemistry
Subjects
Details
- ISSN :
- 14365073 and 00263672
- Volume :
- 183
- Database :
- OpenAIRE
- Journal :
- Microchimica Acta
- Accession number :
- edsair.doi...........53bbcd6c4dea2ef67cfc0728fa7c8316