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Modulation of growth, immunity and antioxidant‐related gene expressions in the liver and intestine of juvenileSillago sihamaby dietary vitamin C
- Source :
- Aquaculture Nutrition. 26:338-350
- Publication Year :
- 2019
- Publisher :
- Hindawi Limited, 2019.
-
Abstract
- An 8‐week feeding experiment was implemented to study the effect of dietary vitamin C (VC) on growth, immunity, oxidation resistance and relevant gene expressions in juvenile S. sihama (2.33 ± 0.02 g). Fish were fed six diets containing VC level of 5, 16, 27, 65, 122 and 233 mg/kg, respectively. VC‐deficient signs including ascites syndrome, skin decay and haemorrhage were found in the fish fed basal diets. Appropriate VC supplement in diets (a) increased weight gain (WG), specific growth rate (SGR), feed intake (FI) and protein efficiency ratio (PER); (b) increased contents of hepatic total immunoglobulin (IgM), complement 3 (C3) and complement 4 (C4) and activity of alkaline phosphatase (AKP); (c) increased hepatic and intestinal copper–zinc superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione S‐transferase (GST) activities, but decreased hepatic MDA content; and (d) up‐regulated gene expressions of NF‐E2‐related factor 2 (Nrf2), Kelch‐like ECH‐associated protein 1 (Keap1), CAT, GST, GPx, glutathione reductase (GR), CuZnSOD and manganese superoxide dismutase (MnSOD) in liver and intestine. These results indicated that dietary VC significantly influenced the growth, immunity and oxidation resistance at enzymatic and genic levels. Based on regression analysis for PER, IgM, intestinal CuZnSOD and hepatic VC concentration, the optimal requirement of dietary VC in juvenile S. sihama was estimated to be 98.33, 139.03, 104.23 and 143.69 mg/kg.
- Subjects :
- chemistry.chemical_classification
0303 health sciences
medicine.medical_specialty
Protein efficiency ratio
Antioxidant
biology
Vitamin C
medicine.medical_treatment
Glutathione peroxidase
Glutathione reductase
04 agricultural and veterinary sciences
Glutathione
Aquatic Science
Superoxide dismutase
03 medical and health sciences
chemistry.chemical_compound
Endocrinology
chemistry
Internal medicine
040102 fisheries
medicine
biology.protein
0401 agriculture, forestry, and fisheries
Alkaline phosphatase
030304 developmental biology
Subjects
Details
- ISSN :
- 13652095 and 13535773
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Aquaculture Nutrition
- Accession number :
- edsair.doi...........5388252989e7c99d455a28cdd3e21c1d