Kinetic theory of biostimulation for azo dye decolorization using immobilized cell system

This kinetic study simulates the characteristics of diazo dye (C.I. Reactive Red 141, RR 141) decolorization using an upflow-packed bed bio-reactor (immobilized cell system, ICS) with indigenous Aeromonas hydrophila entrapped onto the packing matrix, Porites coral. The kinetic theory of bio-reaction in ICS column is found to be the bio-reaction based on Langmuir adsorption isotherm. Our analyses upon two kinds of experimental data reveal that: (1) both ICS data have the first-order and the zero-order asymptotes, two features of reaction with Langmuir adsorption isotherm; (2) all ICS data have a common adsorption equilibrium constant; (3) both the viability of immobilized cells and the ultimate extent of bio-reaction are proportional to the amount of inlet nutrient source; (4) the former is independent of the flow rate of stream; however, the latter is inversely proportional to the flow rate of stream.