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Platelet-activating factor is a downstream messenger of kainate-induced activation of mitogen-activated protein kinases in primary hippocampal neurons
- Source :
- Journal of Neuroscience Research. 53:297-303
- Publication Year :
- 1998
- Publisher :
- Wiley, 1998.
-
Abstract
- Excitatory amino acids transduce physiological and pathological signals to neurons. Similarly, the neuroactive lipid platelet-activating factor (PAF) has been implicated in modulating long-term potentiation and neuronal survival. Excitatory amino acids and PAF have been shown to increase mitogen-activated protein (MAP) kinases in different cell types. Here, we have investigated the similarities and differences between PAF and kainate in activating MAP kinases in primary hippocampal neurons in vitro. Extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38 kinases were activated by kainate or PAF in hippocampal neurons. This activation was blocked by the receptor antagonists CNQX and BN 50730 for kainate and PAF, respectively. The PAF receptor antagonist BN 50730 also blocked kainate activation. CNQX had no effect on PAF activation of the kinases, indicating that PAF is downstream of kainate activation. Coapplication of submaximal concentrations of PAF and kainate resulted in a less than additive activation, suggesting similar routes of activation by the two agonists. Both CNQX and BN 50730 blocked kainate-induced neurotoxicity. These results indicate that PAF and kainate activate similar kinase pathways. Therefore, PAF acts downstream of the kainate subtype of glutamate receptors, and when excessive receptor activation takes place, this bioactive lipid may contribute to neuronal cell death.
- Subjects :
- Platelet-activating factor
Kinase
medicine.drug_class
p38 mitogen-activated protein kinases
Glutamate receptor
Long-term potentiation
Kainate receptor
respiratory system
Biology
Receptor antagonist
Cell biology
Cellular and Molecular Neuroscience
chemistry.chemical_compound
chemistry
Biochemistry
CNQX
medicine
lipids (amino acids, peptides, and proteins)
Subjects
Details
- ISSN :
- 10974547 and 03604012
- Volume :
- 53
- Database :
- OpenAIRE
- Journal :
- Journal of Neuroscience Research
- Accession number :
- edsair.doi...........52525e6ae5e1dab2238f94a679e8ff64