Isolation and 16S rRNA-Based Identification of Benomyl-Degrading Bacteria

Laboratory experiments were conducted to isolate and identify Benlate - (Benomyl) degrading microorganisms from two soil types collected from different locations in Khartoum State, Sudan. Benomyl degradationwas studiedat two temperatures (28 and 40∫C) in soil treated with three Benomyl con centrations (0.032, 3.2 and 8.0 mg Benomyl/g soil) and incubated for 360 days. Potential degraders were also tested in mineral salt liquid medium using Benomyl as a sole carbon source. Degradation percentages were then determined and the most efficient Benomyl degraders were identified by amplification with 16S rRNA gene, sequencing and alignment with deposited sequences in the international gene bank. A total of 64 isolates were recovered from the two soil types, with 59 (92.2%) isolates recovered from the clay soil. Thirty four isolates were recovered from clay soil treated with 8.0mg Ben omyl/g soil and incubated at 28∫ C. The most efficient Benomyl degraders, with degradation percentages in the range of 44 -59, were identified as Pseudomonas stutzeri (Two different isolates) , Pseudomonas putida, Acinetobacter johnsonii, Brevibacillus invocatus, Bacillus clausii, Lysinibacillussp. andAgrobacterium radiobacter.