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The developmental proteome of Drosophila melanogaster
- Source :
- Genome Research. 27:1273-1285
- Publication Year :
- 2017
- Publisher :
- Cold Spring Harbor Laboratory, 2017.
-
Abstract
- Drosophila melanogaster is a widely used genetic model organism in developmental biology. While this model organism has been intensively studied at the RNA level, a comprehensive proteomic study covering the complete life cycle is still missing. Here, we apply label-free quantitative proteomics to explore proteome remodeling across Drosophila’s life cycle, resulting in 7952 proteins, and provide a high temporal-resolved embryogenesis proteome of 5458 proteins. Our proteome data enabled us to monitor isoform-specific expression of 34 genes during development, to identify the pseudogene Cyp9f3Ψ as a protein-coding gene, and to obtain evidence of 268 small proteins. Moreover, the comparison with available transcriptomic data uncovered examples of poor correlation between mRNA and protein, underscoring the importance of proteomics to study developmental progression. Data integration of our embryogenesis proteome with tissue-specific data revealed spatial and temporal information for further functional studies of yet uncharacterized proteins. Overall, our high resolution proteomes provide a powerful resource and can be explored in detail in our interactive web interface.
- Subjects :
- 0301 basic medicine
biology
ved/biology
ved/biology.organism_classification_rank.species
Quantitative proteomics
Computational biology
Proteomics
biology.organism_classification
Transcriptome
03 medical and health sciences
030104 developmental biology
Genetic model
Proteome
Genetics
Drosophila melanogaster
Model organism
Genetics (clinical)
Drosophila Protein
Subjects
Details
- ISSN :
- 15495469 and 10889051
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Genome Research
- Accession number :
- edsair.doi...........51de81900cf67c66a9c202cc44721b27
- Full Text :
- https://doi.org/10.1101/gr.213694.116