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Determination of Cellular Localization of Expression of Flavin-Containing Monooxygenase Genes in Mouse Tissues by In Situ Hybridization
- Publication Year :
- 2006
- Publisher :
- Humana Press, 2006.
-
Abstract
- Methods are described for the cellular localization of expression of flavin-containing monooxygenase (FMO) genes in various mouse tissues by in situ hybridization. These include the production of digoxigenin (DIG)-labeled antisense and sense RNA probes by transcription from FMO cDNA templates, the preparation of paraffin wax-embedded and cryostat tissue sections, the hybridization of RNA probes to tissue sections, and the specific detection of hybridized probes using an antibody to DIG.
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi...........518b1fdd489deb4277f274b9d9fd4283