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Antiproliferative Effect and Cell Cycle Modulation by Melatonin on GH3 Cells
- Source :
- Hormone Research in Paediatrics. 53:251-255
- Publication Year :
- 2000
- Publisher :
- S. Karger AG, 2000.
-
Abstract
- We have investigated the effect of melatonin on cell proliferation and modulation, in the GH3 experimental rat pituitary cell line; the expression of oncogenes c-myc, c-jun and the tumor suppressor gene p53 were also analyzed basally and after exposure to melatonin (10–6, 10–8 and 10–10 M). Melatonin exhibited an antiproliferative effect at all the doses tested, decreasing the proliferating index by 50%. After exposure to melatonin, a decrease in Ki67 and Proliferation cell nuclear antigen occurred acute- and transiently (at 2 h) after a single dose which recovered at 4 h, as well as chronically after repeated 12-hour doses which persisted at 48 h; a similar behavior was observed both acute- and chronically for c-myc and c-jun, while it was opposite for p53, rising acute- and transiently as well as after repeated exposure. These results demonstrate that melatonin modulates the proliferation mechanisms of the GH3 cells.
- Subjects :
- endocrine system
endocrine system diseases
Cell growth
Chemistry
Endocrinology, Diabetes and Metabolism
c-jun
Cell biology
Rat Pituitary
Melatonin
Endocrinology
Cell culture
Pediatrics, Perinatology and Child Health
Cancer research
medicine
Antiproliferative effect
hormones, hormone substitutes, and hormone antagonists
Cell cycle modulation
medicine.drug
Subjects
Details
- ISSN :
- 16632826 and 16632818
- Volume :
- 53
- Database :
- OpenAIRE
- Journal :
- Hormone Research in Paediatrics
- Accession number :
- edsair.doi...........5139af2561f8560d1a7fd878968b211b