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High‐efficiency Ni 2+ ‐NTA/PAA magnetic beads with specific separation on His‐tagged protein
- Source :
- IET Nanobiotechnology. 14:67-72
- Publication Year :
- 2019
- Publisher :
- Institution of Engineering and Technology (IET), 2019.
-
Abstract
- To effective capture and universal enrichment of His-tagged protein, polyacrylic acid (PAA) brushes were used to encapsulate Fe3O4 nanoparticles, connect NTA, and Ni2+ to prepare magnetic beads. These materials provide many advantages, such as excellent stability, tuneable particle size, and a surface for further functionalisation with biomolecules. His-tagged green fluorescence protein (GFP) was separated efficiently, and the binding capacity of Fe3O4/MPS@PAA/NTA-Ni2+ was 93.4 mg/g. Compared with High-Affinity Ni-NTA Resin and Ni-NTA Magnetic Agarose Beads, Fe3O4/MPS@PAA/NTA-Ni2+ nanocomposites exhibited higher separation efficiency and binding capacity towards His-tagged GFP. Moreover, the selectivity and recyclability of them for the target proteins were maintained well after six cycles. This study would widen the application of PAA in constructing multifunctional nanocomposites for biomedical fields.
- Subjects :
- chemistry.chemical_classification
Nanocomposite
Biomolecule
Polyacrylic acid
Nanoparticle
02 engineering and technology
Polymer
biochemical phenomena, metabolism, and nutrition
010402 general chemistry
021001 nanoscience & nanotechnology
01 natural sciences
Fluorescence
0104 chemical sciences
Electronic, Optical and Magnetic Materials
chemistry.chemical_compound
chemistry
Chemical engineering
Agarose
Electrical and Electronic Engineering
0210 nano-technology
Selectivity
Biotechnology
Subjects
Details
- ISSN :
- 1751875X and 17518741
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- IET Nanobiotechnology
- Accession number :
- edsair.doi...........5111f45e0fd55b6dbca573acef083a54