Morphological and molecular identification of airborneFusariumpropagules trapped in a maize field in Hungary

Airborne propagules of Fusarium spp. were collected on Fusarium selective medium with Andersen sampler in maize field. Fusarium isolates were identified based on morphological characters and using speciesspecific primersVERT 1/2, PRO 1/2 and SUB 1/2. The PCR analysis confirmed morphological identification of F. verticillioides, F. proliferatum and F. subglutinans. The VERTF 1/2 set of primers were used to discriminate potential fumonisin-producing strains of F. verticillioides, and all F. verticillioides strains scored positive for VERTF 1/2 pair of primers. This is the first study where specific primers were used for the confirmation of morhological determination of the above-mentioned Fusarium species, and selection of fumonisin-producing airborne isolates of F. verticillioides in Hungary.