New rRNA primers for the detection of Vibrio anguillarum

In this work, 51 V. anguillarum isolates (42/51 of O1 serotype, 9/51 of O2) obtained from cultivated marine fish by Avsever and Un (2015) were used. These isolates were confirmed with universal primers specific to amiB genes. They were also detected successfully at a rate of 100% with a new primer couple designed according to 16S ribosomal RNA sequence. The new primers were found to have a high sensitivity and specificity but were liable to give a cross-reaction with Vibrio ordalii. The IJA appears exclusively as a peer-reviewed on-line open-access journal at http://www.siamb.org.il/. To read papers free of charge, please register online at registration form. Sale of IJA papers is strictly forbidden.