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Regulation mechanism of triterpenoid components from Prunella asiatica on phase Ⅱ detoxifying enzymes in vitro and in vivo
- Source :
- China Journal of Chinese Materia Medica.
- Publication Year :
- 2012
- Publisher :
- China Journal of Chinese Materia Medica, 2012.
-
Abstract
- To study the effects of triterpenoid components from Prunella asiatica on phase II detoxifying enzymes and protein expression in vitro and in vivo. Normal human bronchial epithelial (NHBE) cell model was used in vitro, and the mouse model of Kunming (KM) mice was used in vivo. CDNB assay was used to measure the activity of GST. NADPH and DCIP was used to detect the activity of NQO1. DTNB colorimetric assay was used to detect GSH. Western blot was use to detect the protein expression of NQO1. We found that triterpenoid components from P. asiatica could increase the activity of GST, NQO1 and GSH in NHBE cells and KM mice. NQO1 protein expression can also be increased in vitro. The study suggests that triterpenoid components from P. asiatica can prevent the lung cancer by regulating the body phase II detoxification enzyme activity and protein expression.
- Subjects :
- biology
medicine.diagnostic_test
DTNB
Glutathione
Enzyme assay
In vitro
chemistry.chemical_compound
Complementary and alternative medicine
Biochemistry
Western blot
chemistry
In vivo
Cell culture
Phase II Detoxification
biology.protein
medicine
Pharmacology (medical)
General Pharmacology, Toxicology and Pharmaceutics
Subjects
Details
- ISSN :
- 10015302
- Database :
- OpenAIRE
- Journal :
- China Journal of Chinese Materia Medica
- Accession number :
- edsair.doi...........4ecce04ab6374bbf53b9f2f4c5b96d76