Biodegradation of pyridine raffinate using bacterial laccase isolated from garden soil

Pyridine raffinate, the residual mixture after the extraction of pyridine, discharges as pollutants from pyridine manufacturing industries. Pyridine and its derivatives are of foremost concern as environmental pollutants. Removal of contaminant or pollutant can be achieved using three kinds of approaches: Physical, Chemical and Biological. Paralleled with other physical and chemical methods such as combustion, photolysis. Biodegradation is a fiscal and environment-friendly alternative. In the present study, bacterial laccase, a multi copper enzyme that uses molecular oxygen to oxidize wide variety of aromatic and non-aromatic compounds, was used for the biodeterioration of pyridine raffinate, sample taken from Jubilant Organosys, Gajraula. Laccase was isolated from the bacterium Pseudomonas fluorescens and found to be an effective method to biodeteriorate pyridine raffinate instead of using harmful chemicals. It was observed that O.D. was reduced (max. 0.2327 at 501 nm) measured by UV/VIS Spectrophotometer taking raffinate as a reference. It signifies the oxidation of compounds present in the effluent that leads to the reduction in C.O.D. and found to be an effective method for the treatment of effluent.