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Somatic Antigens of Streptococcus Group E
- Source :
- Applied Microbiology. 19:823-829
- Publication Year :
- 1970
- Publisher :
- American Society for Microbiology, 1970.
-
Abstract
- Antigenic extracts of Streptococcus group E (SGE) were subjected to fractional ethanol precipitation, block (preparative) electrophoresis, and gel filtration for the purpose of separating the type antigen from the group antigen. Ethanol precipitation was ineffective in separating the substances. Block electrophoresis yielded serologically pure group antigen and a mixture of type and group antigen. Serologically pure type antigen was obtained by gel filtration. In some cases, pure group antigen was also recovered; in others, it was contaminated with type antigen. Gel filtration column effluents of antigenic extracts of SGE serotypes, I, II, III, IV, V and “untypable” isolates, collected from the region in which type antigen was eluted, were studied by paper chromatography and infrared spectrophotometry in an effort to develop a nonserological means of detecting type antigen. Hydrolysates of type antigens or suspect type antigens of serotypes I through V contained l -rhamnose, d -glucose, and several unidentified substances. d -Galactose also was present in hydrolysates of serotypes III and V. Untypable isolates and negative controls contained traces of d -glucose only. The data suggested that serotypes I through V contained a type antigen and that the untypable isolates were devoid of type antigen. Infrared absorbance spectra seemed to support the paper chromatography data.
- Subjects :
- Serotype
Chromatography
General Immunology and Microbiology
Chemistry
Rhamnose
Streptococcus
Size-exclusion chromatography
General Medicine
Precipitin
medicine.disease_cause
General Biochemistry, Genetics and Molecular Biology
Paper chromatography
chemistry.chemical_compound
Antigen
medicine
General Pharmacology, Toxicology and Pharmaceutics
Ethanol precipitation
Subjects
Details
- ISSN :
- 00036919
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Applied Microbiology
- Accession number :
- edsair.doi...........4dede9721d85521e2cdfd9bd22ec480c