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The Caprine Casein-Alpha-S2 Protein Modulates the Molecular Mechanism 2 of Insulin Signal Transduction in Type2 Diabetes Rat
- Source :
- Acta Biochimica Polonica.
- Publication Year :
- 2020
- Publisher :
- Polskie Towarzystwo Biochemiczne (Polish Biochemical Society), 2020.
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Abstract
- This study purpose was to investigate the association of casein-alpha-S2 protein of Caprine milk and molecular mechanismofinsulin signaltransduction in type2 diabetes mellitus (T2DM). The Caprine milk CSN1S2 protein treatment of 0, 375, 750, and 1500mg/kg BW were conducted to the control and T2DM rats. We observed several physiological parameters of all rats. The levels of insulin and TNF-α in the plasma were measured using ELISA.The expressions of proteins and mRNA levels of diabetes-related genes in the pancreas tissues were analyzed using Western Blotting and Real-Time PCR, respectively. Our study found that diabetic rats had lower body weight, food intake, and fecal weight compared with control rats. The Caprine milk CSN1S2 protein consumption affected the body weight of diabetic rats to increase, especially at the dose of 750mg/kg BW.Interestingly, the genes associated with insulin signaling were improved by the CSN1S2 protein treatment in diabetic rats, although their blood glucose and cholesterol level were not affected. The diabetic rats showed an elevated insulin level and GLUT4 protein expression after treatment. We also reported that the CSN1S2-treated diabetic rats had a gradually reduced expression of TNF-α and VCAM-1 in dose-dependent. Moreover, the 750mg/kg BW of CSN1S2 treatment enhanced the mRNA expressions of INS-receptor, GLUT4, IGF-1, CAMKK, and CAMKIV in diabetic rats. The ability of Caprine milk CSN1S2 protein to regulate the molecular mechanisms in the diabetes-signaling pathway indicated its potential therapeutic effects on diabetes management.
- Subjects :
- 0303 health sciences
medicine.medical_specialty
biology
Chemistry
Cholesterol
Insulin
medicine.medical_treatment
030302 biochemistry & molecular biology
Type 2 diabetes
medicine.disease
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Insulin receptor
chemistry.chemical_compound
Endocrinology
Diabetes mellitus
Casein
Internal medicine
biology.protein
medicine
Signal transduction
GLUT4
Subjects
Details
- ISSN :
- 1734154X and 0001527X
- Database :
- OpenAIRE
- Journal :
- Acta Biochimica Polonica
- Accession number :
- edsair.doi...........4d682900dec9b2761f8c79717974d95f