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Fabrication of Novel Polymer Nanoparticle-Based Fluorescence Resonance Energy Transfer Systems and their Tunable Fluorescence Properties
- Source :
- Journal of Macromolecular Science, Part A. 48:219-226
- Publication Year :
- 2011
- Publisher :
- Informa UK Limited, 2011.
-
Abstract
- In this study, two hydrophobic fluorescent dyes, nitrobenzoxadiazolyl (NBD) and 9-(diethylamino)benzo[a]phenoxazin-5-one (NR) with different doping ratios were incorporated into polymer nanoparticles to constitute novel polymer nanoparticle-based fluorescence resonance energy transfer (FRET) systems via a facile one-step mini-emulsion polymerization. Spectroscopic characteristics demonstrate that the two fluorophores have been successfully embedded into the nanoparticles, and the fluorescence emission intensity of the two hydrophobic dyes can be greatly enhanced in aqueous media. The as-prepared fluorescent nanoparticles also display a uniform small size (ca. 55 nm), high dye load, intense fluorescence, as well as controllable amount and ratio of the two dyes. The observed FRET efficiencies (16.0–75.2%), as well as the distance (r) between NBD (donor) and NR (acceptor), is closely correlated to the doping ratio of two dyes. Moreover, by varying the doping ratio of two dyes, the fluorescent nanoparticles w...
- Subjects :
- chemistry.chemical_classification
Resonant inductive coupling
Polymers and Plastics
Chemistry
technology, industry, and agriculture
Nanoparticle
General Chemistry
Polymer
Fluorescence in the life sciences
Photochemistry
Acceptor
Fluorescence
Förster resonance energy transfer
Polymerization
Materials Chemistry
Ceramics and Composites
Subjects
Details
- ISSN :
- 15205738 and 10601325
- Volume :
- 48
- Database :
- OpenAIRE
- Journal :
- Journal of Macromolecular Science, Part A
- Accession number :
- edsair.doi...........4ce558d109dc81e35c2aa27c0b7b660f