Cryopreservation v1

Samples of kidney tissues are cryopreserved, sectioned and processed for various analyses. KPMP biopsy tissue undergoes initial processing (e.g. OCT embedding) at the Recruitment Sites, prior to distribution to Tissue Interrogation Sites. KPMP pilot nephrectomies were cryopreserved at University of Michigan. IU biopsies and nephrectomies were cryopreserved as described below. The tissue is sectioned according to a predetermined order: Bulk, LMD, miRNA, proteomics at 12 µm and then 50 µm sections for imaging studies as described in Section 4.4. This allows efficient use of the edge tissue and maximization of imaging data that will anchor the LMD information with the remainder of the biopsy to be sent to other Tissue Interrogation Sites for further interrogation. Shared tissue blocks received from other Tissue Interrogation Sites will be sectioned in the reverse order.