Back to Search Start Over

ATR-FTIR Analysis of Amyloid Proteins

Authors :
Vincent Raussens
Jean Marie Ruysschaert
Source :
Methods in Molecular Biology ISBN: 9781493978090
Publication Year :
2018
Publisher :
Springer New York, 2018.

Abstract

Attenuated total reflection FTIR (ATR-FTIR) has been used for decades to study protein secondary structures. More recently, it reveals also to be an exquisite and sensitive tool to study and discriminate amyloid aggregates. Based on the analysis of specific spectral features of β-sheet structures, we present here a detailed protocol to differentiate oligomers vs. fibrils. This protocol, applicable to all amyloid proteins, demonstrates the power of this inexpensive, rapid, and low protein material-demanding method.

Details

ISBN :
978-1-4939-7809-0
ISBNs :
9781493978090
Database :
OpenAIRE
Journal :
Methods in Molecular Biology ISBN: 9781493978090
Accession number :
edsair.doi...........4bcd958ef6a1dbb4f927f57c19191b1a
Full Text :
https://doi.org/10.1007/978-1-4939-7811-3_3