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Protective effect of i Zizyphus spinachristi i on MPP -induced oxidative stress
- Source :
- Frontiers in Bioscience. 10:285-299
- Publication Year :
- 2018
- Publisher :
- IMR Press, 2018.
-
Abstract
- Oxidative stress and mitochondrial dysfunction mediated neuro apoptosis is reported to play a major role in the pathology of Parkinson's disease. Zizyphus spina-christi fruits (ZSCF) are used as traditional medicines that are well-known for their high antioxidant properties. In the present study, we investigated the protective effects of ZSCF extract against 1-methyl-4-phenylpyridinium (MPP+) induced neurotoxicity in SH-SY5Y cell lines. The effect of ZCSF on MPP+ induced cell viability (MTT - 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay), membrane damage - (lactate dehydrogenase (LDH), oxidative stress (levels of ROS, nitric oxide and GSH and activities of SOD and catalase), mitochondrial membrane potential and apoptosis (activity of caspase 3 and protein expressions of cyto c, Bax and Bcl-2) were measured. Our results showed that ZSCF could be able to reduce the neurotoxicity of MPP+ and offer neuroprotection in vitro. This protective effect of ZCF might be mediated by its potent antioxidant properties. However, further research is necessary to isolate active compounds and performing preclinical and clinical studies to confirm the neuro-protective effects of ZSCF in PD.
- Subjects :
- 0301 basic medicine
General Immunology and Microbiology
biology
Chemistry
Neurotoxicity
Caspase 3
Glutathione
Pharmacology
medicine.disease
medicine.disease_cause
Neuroprotection
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
0302 clinical medicine
Catalase
Apoptosis
biology.protein
medicine
Viability assay
030217 neurology & neurosurgery
Oxidative stress
Subjects
Details
- ISSN :
- 19450524 and 19450516
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Frontiers in Bioscience
- Accession number :
- edsair.doi...........4ac4bfcbba15525526756ed24023976c