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Effects of Short Chain Fatty Acids (SCFAs) Modulation on Potentially Diarrhea Causing Pathogens in Yaks Through Metagenomics Sequencing

Effects of Short Chain Fatty Acids (SCFAs) Modulation on Potentially Diarrhea Causing Pathogens in Yaks Through Metagenomics Sequencing

Authors :
Kun Li
Muhammad Shahzad
Aoyun Li
Juanjuan Liu
Yaping Wang
Lulu Pei
Jiakui Li
Zhibo Zeng
Muhammad Fakhar-e-Alam Kulyar
Desheng Qi
Publication Year :
2020
Publisher :
Research Square Platform LLC, 2020.

Abstract

Yaks are of great importance on the plateau; however, an emerging endemic diarrheal disease during the last few years is posing a great threat to the health of these animals. Yaks have special gut microbiotal community and short-chain fatty acids (SCFAs) which are not only the principle nutrient substrates of intestinal epithelial cells but can also regulate the epithelial barrier. Until now, metagenomics sequencing has not been reported in diarrheal yaks. A scarce information is available regarding the levels of fecal SCFAs and diarrhea in yaks. The purpose of our study was to identify the potential pathogens that cause the emerging diarrhea and also to explore the potential relationship of short-chain fatty acids in this issue. We estimated diarrhea rate in yaks after collecting the equal number of fecal samples from affected animals. Metagenomics sequencing and quantitative analysis of SCFAs were performed which revealed 15-25% and 5-10% prevalence in diarrheal yak’s calves and adults yaks respectively. Significant difference was observed in GC contents (44.69%~46.08% vs 46.12%~46.38%) under two reference groups (ppppppppppppStaphylococcus aureus, Babesia ovata, Anaplasma phagocytophilum, Bacteroides fluxus, viruses, Klebsiella pneumonia, and inflammation-related bacteria; the decreased of SCFAs caused by the imbalance of intestinal microbiota may potentially leads to emergence of diarrhea in yaks.

Details

Database :
OpenAIRE
Accession number :
edsair.doi...........49a45215df7af511f93745d24f4cd88a
Full Text :
https://doi.org/10.21203/rs.3.rs-91179/v1