Chemically Defined Medium for Optimization of Proteolytic Activity of Lactobacillus bulgaricus 761N

casein content. CaCl2 and CoCl2 were found to enhance the proteolytic activity at 1mM, while CuSO4 and FeSO4 were found to be needed in fewer amounts at 0.5mM and 0.1mM, respectively. The antibacterial activity of these bioactive peptides produced in the cell free supernatant was investigated against some human pathogenic bacteria. It was found that it impose a high antibacterial capacity on tested pathogens reaching 89% inhibition.