SIMULTANEOUS DETERMINATION OF HYPOXANTHINE, XANTHINE AND URIC ACID USING AN IMMOBILIZED XANTHINE OXIDASE BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

Immobilized xanthine oxidase was prepared by intermolecular cross-linking to porous aminopropyl-glass beads, and the properties were investigated in a batch process. Simultaneous analysis of hypoxanthine, xanthine and uric acid was achieved by using small column packed with the immobilized xanthine oxidase as a reactor for high-performance liquid chromatography (HPLC).