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Sensitivity of Na+/K+-ATPase isoforms to acid and aluminum explains differential effects on Atlantic salmon osmoregulation in fresh water and seawater
- Source :
- Canadian Journal of Fisheries and Aquatic Sciences. 75:1319-1328
- Publication Year :
- 2018
- Publisher :
- Canadian Science Publishing, 2018.
-
Abstract
- Atlantic salmon (Salmo salar) smolts are sensitive to acid rain and associated increases in dissolved inorganic aluminum (Al) resulting in decreased seawater tolerance at this critical life stage. Salmon have two major isoforms of the catalytic alpha subunit of Na+/K+-ATPase (NKA), with NKAα1a being the major freshwater (FW) isoform and NKAα1b the major seawater (SW) isoform. Here we evaluate physiological markers of SW preparedness and NKAα1a and NKAα1b isoforms after short-term exposure to acidified water and acidified water with added Al (acid–Al). Atlantic salmon smolts were exposed to low ion FW (Control), low ion acidic water (pH 5.2; acid), and low ion acidic water (pH 5.2) with moderate levels of added inorganic Al (35 μg·L−1; acid–Al) for 4 days. Acid exposure resulted in loss of salinity tolerance (higher plasma chloride (Cl) after SW exposure) and significantly decreased the levels of gill NKAα1b but not NKAα1a protein abundance. Acid–Al exposure resulted in loss of plasma Cl in FW and higher plasma Cl in SW and decreased NKAα1a and NKAα1b abundance. The loss of salinity tolerance in smolts can be explained by the differential sensitivity of NKA isoforms to acid.
- Subjects :
- 0106 biological sciences
Gene isoform
biology
Chemistry
010604 marine biology & hydrobiology
010501 environmental sciences
Aquatic Science
biology.organism_classification
01 natural sciences
Salinity
Environmental chemistry
Osmoregulation
Seawater
Acid rain
Salmo
Na+/K+-ATPase
Ecology, Evolution, Behavior and Systematics
0105 earth and related environmental sciences
G alpha subunit
Subjects
Details
- ISSN :
- 12057533 and 0706652X
- Volume :
- 75
- Database :
- OpenAIRE
- Journal :
- Canadian Journal of Fisheries and Aquatic Sciences
- Accession number :
- edsair.doi...........4829364bcee4b3ff247c2fe5743da8c8