Additional file 4: Figure S1. of Comparative analyses of glycerotoxin expression unveil a novel structural organization of the bloodworm venom system

SDS-PAGE of reduced G. tridactyla venom. Figure S2. Genomic organization of the glycerotoxin gene analyzed in G. tridactyla. Figure S3. Immunolocalization of GLTx in a cross section through a putative venom gland embedded in paraffin. Figure S4. Anti-serotonin (5-HT) staining and phalloidinâ rhodamine counterstaining on everted G. tridactyla pharynges cut into two halves. Figure S5. Quantitative real-time PCR (qPCR) expression levels (shown as Fold Change, RQ) between biological groups (putative venom glands [A], pharyngeal lobes [B], and posterior body wall [C]) per analyzed specimen (nâ =â 10, biological samples). Figure S6. Tissues analyzed in comparative GLTx expression studies (qPCR experiments and transcriptome analyses) on G. tridactyla. (PDF 2931 kb)