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Degradation of neonicotinoid insecticide acetamiprid by two different nitrile hydratases of Pseudaminobacter salicylatoxidans CGMCC 1.17248
- Source :
- International Biodeterioration & Biodegradation. 157:105141
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Acetamiprid is a neonicotinoid insecticide used worldwide that has caused environmental pollution and adverse effects on ecosystems. Here, a novel bacterium, Pseudaminobacter salicylatoxidans CGMCC 1.17248, was isolated to rapidly degrade acetamiprid to IM-1-2 via hydration. Gene cloning and overexpression demonstrated that two nitrile hydratases, AnhA and AnhB, converted acetamiprid to IM-1-2. Escherichia coli overexpressing AnhA and AnhB degraded 98.1% and 94.0% of acetamiprid (1.0 mmol L−1) in 5 min and 8 h, respectively. The pure AnhA and AnhB had a Vmax value of 14.12 and 1.20 μmol mg−1 min−1, respectively, and a Km value of 1.02 mmol L−1 and 2.95 mmol L−1, respectively. Compared with AnhA, AnhB had broad pH stability, as well as metal ions and organic solvents tolerance. Expression of AnhA and AnhB was induced by decreasing the nutrient concentration of culture broth and addition of urea and therefore significantly enhanced acetamiprid degradation of CGMCC 1.17248. qPCR indicated that the expression of AnhA and AnhB under the cultured conditions of 1/15 lysogeny broth or 0.5% urea addition was improved by from 2.2 to 5.3 times. The results presented herein will facilitate development of bioremediation agents for acetamiprid pollution and understanding of the functional role of bacterial nitrile hydratase.
- Subjects :
- 0301 basic medicine
030106 microbiology
Environmental pollution
010501 environmental sciences
medicine.disease_cause
01 natural sciences
Microbiology
Acetamiprid
Biomaterials
03 medical and health sciences
chemistry.chemical_compound
Nitrile hydratase
Lysogeny broth
medicine
Food science
education
Waste Management and Disposal
Escherichia coli
0105 earth and related environmental sciences
education.field_of_study
biology
Chemistry
biology.organism_classification
Pseudaminobacter salicylatoxidans
Urea
Bacteria
Subjects
Details
- ISSN :
- 09648305
- Volume :
- 157
- Database :
- OpenAIRE
- Journal :
- International Biodeterioration & Biodegradation
- Accession number :
- edsair.doi...........47898e8599f052fe057fab505b8affdb