From specialized cell cultures to high quality fine chemicals: ANTHOcyanin production PLatform Using Suspension cultures (ANTHOPLUS)

ANTHOPLUS yields anthocyanins in stable plant tissue cultures. We aim to produce purified anthocyanins, of novel complexity in side chain decoration, or labelled with stable isotopes for assaying the composition of feedstock for natural colors, bioavailability, bioefficacy and mechanistic research in experimental medicine and as analytical standards. Production comprises the whole value-chain from synthesis, scale-up, preparative purification, assessment of toxicity and bioactivities to distribution for sales. We focus on strategies established for the medium-scale purification of anthocyanins from cell suspension cultures. Anthocyanin profiles of plant cell extracts and chemical structures of candidate molecules are analyzed by LC-MS. Selected compounds are purified by sequential flash and preparative reversed phase chromatography. The purity of resulting fractions is validated by a combination of LC-MS and GC-MS approaches, as well as by enzyme assays.
Journal of International Society of Antioxidants in Nutrition & Health, Vol 3, No 4 (2016)