Fabrication of amyloid peptide micro‐arrays using laser‐induced forward transfer and avidin‐biotin mediated assembly

Peptide-based arrays and patterns are a powerful tool in the study of protein functionality, with a variety of applications such as the study of interactions between peptides and enzymes, proteins and DNA. One of the main and most critical issues is the generation of high density arrays which maintain the biological function of the peptides. In this study, we employ Laser-Induced Forward Transfer for the generation of high density photosensitive biotin arrays on ORMOCER®-coated surfaces. We then generate peptide microarrays, which assemble due to the employment of avidin-biotin and thiol chemistry. These initial results are poised to the construction of larger peptide patterns as scaffolds for the incorporation and display of ligands critical for cell attachment and growth, or for the templating of inorganic materials. (© 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)