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Because little is known about filamentous fungal tensile strength, it is assumed to be constant in many models. A method involving off-line fragmentation and image analysis is used now to measure relative tensile strength of Aspergillus oryzae hyphae. During the course of shake-flask and fed-batch cultures, hyphal tensile strength increases during growth then decreases. Hyphal tensile strength can change up to 3-fold over time.