Additional file 2: of CSF H3F3A K27M circulating tumor DNA copy number quantifies tumor growth and in vitro treatment response

Figure S1. Serial dilution of K27M mutant oligonucleotide in constant background of wild-type DNA demonstrates consistent detection down to at least 2% VAF under typical experimental conditions, with the possibility of detection at even lower VAF under ideal conditions. One such dilution series is shown above, with (a) showing number of droplets positive for mutant or wild-type H3F3A sequence and (b) showing the corresponding VAF values. Figure S2. Plot of droplets (blue â positive mutant H3F3A K27M, green â positive wildtype H3F3A, grey â negative droplets) from ddPCR performed on (a) non-tumor human CSF spiked with synthetic K27M mutant sequence oligonucleotide and (b) non-tumor human CSF alone. (DOCX 268 kb)