Functional actin cytoskeleton is required in early stage of NETosis induction

Neutrophil extracellular trap formation (NETosis) is a dynamic process featuring nuclear chromatin extrusion and extracellular trap formation (1) in which ruptures of nuclear envelope and plasma membrane are prerequisite events. Thiam et al. (1) analyze several important cellular events, including cytoskeleton organization, during NETosis using time-lapse microscopy. They conclude that NETosis begins with rapid disassembly of actin cytoskeleton, based on the microscopy analysis of a few hundred neutrophils (1). However, other studies have found that pharmacologic (2, 3) or genetic (4) inhibition of actin assembly decreases NET formation, indicating the role of functional actin cytoskeleton in NETosis induction. To systematically explore the dynamic changes of actin, our time course study with flow cytometry analysis … [↵][1]1Email: lium1{at}pennmedicine.upenn.edu. [1]: #xref-corresp-1-1