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Age-related vulnerability to nigral dopaminergic degeneration in rats via Zn2+-permeable GluR2-lacking AMPA receptor activation
- Source :
- NeuroToxicology. 83:69-76
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- On the basis of the evidence that extracellular Zn2+ influx induced with AMPA causes Parkinson's syndrome in rats that apomorphine-induced movement disorder emerges, here we used a low dose of AMPA, which does not increase intracellular Zn2+ level in the substantia nigra pars compacta (SNpc) of young adult rats, and tested whether intracellular Zn2+ dysregulation induced with AMPA is accelerated in the SNpc of aged rats, resulting in age-related vulnerability to Parkinson's syndrome. When AMPA (1 mM) was injected at the rate of 0.05 μl/min for 20 min into the SNpc, intracellular Zn2+ level was increased in the SNpc of aged rats followed by increase in turning behavior in response to apomorphine and nigral dopaminergic degeneration. In contrast, young adult rats do not show movement disorder and nigral dopaminergic degeneration, in addition to no increase in intracellular Zn2+. In aged rats, movement disorder and nigral dopaminergic degeneration were rescued by co-injection of either extracellular (CaEDTA) or intracellular (ZnAF-2DA) Zn2+ chelators. 1-Naphthyl acetyl spermine (NASPM), a selective blocker of Ca2+- and Zn2+-permeable GluR2-lacking AMPA receptors blocked increase in intracellular Zn2+ in the SNpc of aged rats followed by rescuing nigral dopaminergic degeneration. The present study indicates that intracellular Zn2+ dysregulation is accelerated by Ca2+- and Zn2+-permeable GluR2-lacking AMPA receptor activation in the SNpc of aged rats, resulting in age-related vulnerability to Parkinson's syndrome.
- Subjects :
- inorganic chemicals
0303 health sciences
medicine.medical_specialty
Pars compacta
General Neuroscience
Dopaminergic
Spermine
Substantia nigra
AMPA receptor
Toxicology
Apomorphine
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
nervous system
chemistry
Internal medicine
medicine
Extracellular
030217 neurology & neurosurgery
Intracellular
030304 developmental biology
medicine.drug
Subjects
Details
- ISSN :
- 0161813X
- Volume :
- 83
- Database :
- OpenAIRE
- Journal :
- NeuroToxicology
- Accession number :
- edsair.doi...........455816db83eb0a555ef3f96cc19ddf43