First Report of Chilli veinal mottle virus Infecting Lycopersicon esculentum in Jiangsu Province in China

Chilli veinal mottle virus (ChiVMV) belongs to the genus Potyvirus, family Potyviridae, infects mostly Capsicum sp. and is aphid-transmitted in a non-persistent manner in the field. This virus is widespread and has been reported in Hainan Province (Wang et al. 2006), Yunnan Province (Ding et al. 2011), and Sichuan Province (Zhao et al. 2014) in China. In June 2016, virus-like symptoms such as mottling, mosaic, and narrowing were observed on tomato (Lycopersicon esculentum) plants in Yangzhou city, Jiangsu Province, east China. Five leaf samples were collected from symptomatic tomato plants. All samples were assayed by western blotting using polyclonal antiserum to ChiVMV. The results suggest that two of five samples were positive for ChiVMV. Total RNA was extracted from 5 symptomatic tomato plants of leaf samples using Trizol reagent (Invitrogen, Carlsbad, CA), according to the manufacturer’s protocol. The first-strand cDNA was prepared using M-MLV Reverse Transcriptase (Invitrogen, Carlsbad, CA). PCR was...