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Smart phone-powered capillary electrophoresis on a chip for foodborne bacteria detection
- Source :
- Sensors and Actuators B: Chemical. 301:127108
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Due to the requirement of high-voltage power supply, on-chip micro-capillary electrophoresis (μCE) suffers from practical on-site bioapplications. In this paper, we introduced a smart phone based electric power system for μCE on a chip with simple electrical operations and improved portability of a μCE device. Since the power of the smart phone is limited to 5 V, we utilized a boost converter circuit to increase up to 250 V, which enables μCE on a chip. The typical μCE channel with a cross design was patterned on the PMMA substrate, and the sample, the waste, the anode and the cathode reservoirs were fabricated. Two steps of μCE consisting of the injection and the separation were performed to separate two amplicons (121 bp FliC gene of Escherichia coli and 161 bp InvA gene of Salmonella serovar Typhimurium). During the injection step, the voltages applied in the sample and waste reservoirs were 0 V and 250 V, respectively, and the amplicons were filled in the injection channel (6.8 mm length) in 50 s. We switched the power supply to the anode reservoir with 250 V and the cathode reservoir with 0 V. Two amplicons were successfully separated in the separation channel (17 mm length) in 4 min. In order to assign the peak in the electropherogram, we added two bracket ladders with the amplicon sample, so that we could accurately identify the target peak based on a relative migration time.
- Subjects :
- Materials science
02 engineering and technology
010402 general chemistry
01 natural sciences
law.invention
Electric power system
Capillary electrophoresis
law
Materials Chemistry
Electrical and Electronic Engineering
Instrumentation
business.industry
Metals and Alloys
021001 nanoscience & nanotechnology
Condensed Matter Physics
Chip
Cathode
0104 chemical sciences
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Anode
Electropherogram
Boost converter
Optoelectronics
0210 nano-technology
business
Voltage
Subjects
Details
- ISSN :
- 09254005
- Volume :
- 301
- Database :
- OpenAIRE
- Journal :
- Sensors and Actuators B: Chemical
- Accession number :
- edsair.doi...........45009981730aa9bc8583301bef281233